Impact of intestinal bacteria on DNA methylation and tumorigenesis

We utilized two different groups of GF Apc Min/+ mice and colonized them with two differentconsortia, one harboring a pks + E. coli strain with "low-pks" activity (DSMZ) and the second with aclinical isolate exhibiting "high-pks" activity (UM149). Mice were colonized with the differentconsortia, exposed to DSS-induced colitis, and analyzed for tumor burden, DNA methylation, andtranscriptional changes.