Differentially expressed genes from two groups of Tribolium castaneum selected for short or long duration of death feigning behaviour
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The dataset presented here consists of a spreadsheet in Excel .xlsx format, containing information on differentially expressed genes (DEGs) from two groups of the red flour beetle, <i>Tribolium castaneum</i>; one selected for a short duration (S) and the other a long duration (L) of death feigning antipredator behaviour.<br>An RNA-seq analysis was performed on the S and L strains of <i>T. castaneum </i>to elucidate the relationship between gene expression and differences in death feigning behaviour. cDNA sequencing was performed using the Illumina HiSeq 2500 platform. Approximately 90% of the clean reads obtained were mapped to the Tcas5.2 reference genome, and further analysis revealed 518 DEGs between the L and S strains. Information on the DEGs identified is presented here in the file <b>death-feigning-degs.xlsx</b>. The information provided is as follows:<br><b>LOC name</b> - Gene symbol for the gene identified<b>Region </b>- Genomic region of the gene identified<b>Gene ID</b> - ID of the gene identified<b>Description</b> - Gene description<b>FPKM for L</b> - Fragments Per Kilobase per Million mapped reads value for the L strain<b>FPKM for S</b> - Fragments Per Kilobase per Million mapped reads value for the S strain<b>log2(Fold change+1)</b><br><b>p-value</b><b>FDR</b> - False Discovery Rate<br>These results are further discussed and presented in the figures of the accompanying paper.
本数据集采用Excel .xlsx格式的电子表格存储，包含两类赤拟谷盗（Tribolium castaneum）的差异表达基因（differentially expressed genes, DEGs）信息：一类经选育后表现出短时（S组）死亡拟态反捕食行为，另一类则表现出长时（L组）该行为。 针对赤拟谷盗的S、L品系开展RNA测序（RNA-seq）分析，以解析基因表达与死亡拟态行为差异之间的关联。实验采用Illumina HiSeq 2500平台完成cDNA测序。所得清洁读段中约90%可比对至Tcas5.2参考基因组，后续分析共鉴定出518个L与S品系间的差异表达基因。 本数据集提供的已鉴定DEGs信息存储于文件**death-feigning-degs.xlsx**中，具体包含以下内容： **LOC号** - 所鉴定基因的基因符号 **基因组区域** - 所鉴定基因的基因组定位区域 **基因编号（Gene ID）** - 所鉴定基因的唯一标识 **基因功能描述** - 对应基因的注释信息 **L组FPKM值** - L品系的每百万比对读段每千碱基片段数（Fragments Per Kilobase per Million mapped reads, FPKM） **S组FPKM值** - S品系的每百万比对读段每千碱基片段数 **log₂(倍数变化+1)** **p值（p-value）** **FDR** - 错误发现率（False Discovery Rate） 上述研究结果已在配套论文的相关图表中进行了进一步讨论与展示。
