Heatmap of regional expression OPRM1 splice variant mRNAs.

The heatmap was generated by clustering Z-scores calculated from the values (Log2 (E−ΔC(t)) of individual variants across different strains/regions using R statistical language (2.15.0) (www.r-project.org), and plotting based upon individual brain regions within each mouse strain with enhanced heatmap function heatmap.2 from gplots package. Pfc: prefrontal cortex; Str: striatum; Tha: thalamus; Hyp: hypothalamus; Hip: hippocampus; PAG: periaqueductal gray; BS: brainstem; Cb: cerebellum; Spc: spinal cord; WB: whole brain. 129∶129P3/J; B6: C56BL/6J; SJL: SJL/J; SWR: SWR/J. Expression level was indicated by Z-score.本数据集的热图通过以下方式生成：首先针对不同品系/区域的个体变异的Log₂(E−ΔC(t))值计算得到Z分数，随后借助R统计语言（2.15.0版本，官网地址www.r-project.org）对该Z分数进行聚类，并调用gplots扩展包中的增强型热图函数heatmap.2，以各小鼠品系内的单个脑区为单位进行绘图；本次实验涉及的脑区缩写对应如下：Pfc：前额叶皮层（prefrontal cortex）、Str：纹状体（striatum）、Tha：丘脑（thalamus）、Hyp：下丘脑（hypothalamus）、Hip：海马体（hippocampus）、PAG：中脑导水管周围灰质（periaqueductal gray）、BS：脑干（brainstem）、Cb：小脑（cerebellum）、Spc：脊髓（spinal cord）、WB：全脑（whole brain）；小鼠品系缩写对应为：129：129P3/J、B6：C56BL/6J、SJL：SJL/J、SWR：SWR/J；本数据集中的表达水平以Z分数进行表征。