Tl amd dl mediate the Aβ42 induced rough eye phenotype.

The rough eye phenotype of pGMR-Aβ42 flies was assigned a value of ++++. According to this scoring system, strong, moderate and weak suppression corresponded to +, ++, and +++ respectively. Weak and moderate enhancement corresponded to +++++ and ++++++. All flies are hemizygous for the transgene and either wild type (+) or heterozygous for the indicated mutation. In the experiment with the hsp83:dl transgene the flies are hemizygous for this transgene.将pGMR-Aβ42果蝇的粗糙眼表型赋值为++++。根据该评分体系，强抑制、中度抑制与弱抑制分别对应+、++与+++。弱增强与中度增强则分别对应+++++与++++++。所有受试果蝇均为该转基因的半合子，且要么为野生型（+），要么携带所标注突变的杂合基因型。在开展携带hsp83:dl转基因的实验时，受试果蝇为该转基因的半合子。