Gene expression analysis during transcription factor Ascl1 induced astrocyte-to-neuron conversion. Mus musculus

To investigate the molecular mechanisms underlying transcription factor Ascl1 induced astrocyte-to-neuron conversion, we infected the cultured dorsal midbrain astrocytes with FUGW-Ascl1 or control viruses and performed RNA sequencing (RNA-seq) assays at 2 days post infection (DPI) and 5 DPI with five groups of samples including day0-A (astrocytes control without virus infection at day 0), day2-Ctrl and day5-Ctrl (astrocytes infected with FUGW at day 2 and day 5), day2-Ascl1 and day5-Ascl1 (astrocytes infected with FUGW-Ascl1 at day 2 and day 5). Two biological replicates were used for the RNA-seq. Total RNA was isolated with Trizol reagent. Libraries were prepared, and sequencing was performed in 100-bp paired-end format on the Illumina HiSeq 2000.