Overview of the transmission experiment with unvaccinated birds (high infection dose)*.

*the infection dose is 0.2 ml of 106 EID50 intranasally (0.1 ml) and intraocularly (0.1 ml).i: inoculated bird; c: contact bird; x/y: test result for virus isolation in the trachea/cloaca.?the bird died.#the bird was alive at the end of the experiment and tested negative in the serological test.*感染剂量为10^6 EID50（鸡胚半数感染量）的0.2毫升制剂，经鼻内（0.1毫升）与眼内（0.1毫升）途径联合接种。 i：接种受试禽；c：接触暴露受试禽；x/y：气管/泄殖腔病毒分离检测结果。 该受试禽死亡。 该受试禽在实验结束时仍存活，且血清学检测结果为阴性。