Relative expression of fourteen honey bee genes as measured by RNA-seq and qPCR.

*PThe fourteen genes are a subset of the 81 genes identified as differentially expressed by RNA-seq. The first two columns of data are technical replicates of the same biological sample, whereas the latter two columns represent a different cohort and include an additional time point. P-values are indicated for the two-sided t-test of equal means ΔCt in treatment and control groups, where ΔCt is the approximate expression differential on a log2 scale relative to the geometric mean of five reference genes. T-tests were performed on the log2 values because the linear values deviate strongly from a normal distribution. Expression cluster is that assigned to the best Drosophila melanogaster homolog of each gene by [30]; “n/a” indicates that no Drosophila homolog exists and “none” indicates that the Drosophila homolog was left unclustered by [30].* 本次分析涉及的14个基因为经RNA测序（RNA-seq）鉴定得到的81个差异表达基因的子集。数据的前两列为同一生物学样本的技术重复，而后两列则来自另一独立队列，且包含额外的时间点样本。本研究给出了处理组与对照组中ΔCt均值相等的双侧t检验P值；其中ΔCt为以5个内参基因的几何均值为参照的log₂尺度近似表达差异值。由于原始线性表达值严重偏离正态分布，故对log₂转换后的数据进行t检验。每个基因的表达聚类注释由文献[30]基于其最优黑腹果蝇（Drosophila melanogaster）同源基因给出；其中"n/a"表示不存在对应的黑腹果蝇同源基因，"none"表示该黑腹果蝇同源基因未被文献[30]进行聚类注释。