Details of GEO datasets used in the study.

Hidradenitis suppurativa (HS) is a chronic inflammatory skin disorder, affecting the pilosebaceous unit in apocrine gland-rich areas, characterized by painful nodules, abscesses and draining tunnels. The underlying molecular and immunological mechanisms remain poorly understood. This study aimed to identify key gene expression patterns, hub genes, and analyze the potential role of the CCL19/CCL21-CCR7 axis in HS lesions and peripheral blood using bulk and single-cell RNA sequencing analyses. By employing an integrative approach that included three machine learning methods and subsequent validation on an independent dataset, we successfully identified AKR1B10, IGFL2, WNK2, SLAMF7, and CCR7 as potential hub genes and therapeutic targets for HS treatment. Furthermore, our study found that CCL19 and CCL21 may originate from various cells such as fibroblasts and dendritic cells, playing a crucial role in recruiting CCR7-associated immune cells, particularly Treg cells. The involvement of the CCL19/CCL21-CCR7 axis in HS pathogenesis suggests that other CCR7-expressing cells may also be recruited, contributing to disease progression. These findings significantly advance our understanding of HS pathogenesis offer promising avenues for future CCR7-targeted therapeutic interventions.化脓性汗腺炎（Hidradenitis suppurativa, HS）是一种慢性炎症性皮肤病，主要累及顶泌汗腺富集区域的毛囊皮脂腺单位，以疼痛性结节、脓肿及引流窦道为特征性临床表现，其潜在分子与免疫学发病机制目前仍未被充分阐明。本研究旨在通过批量RNA测序（bulk RNA sequencing）与单细胞RNA测序分析，明确HS皮损及外周血中的关键基因表达模式与核心基因，并解析CCL19/CCL21-CCR7轴在HS病变及外周血中的潜在作用。本研究采用整合了三种机器学习方法的分析策略，并在独立数据集上完成验证，最终成功鉴定出AKR1B10、IGFL2、WNK2、SLAMF7及CCR7作为HS治疗的潜在核心基因与治疗靶点。此外，本研究发现CCL19与CCL21可由成纤维细胞、树突状细胞等多种细胞类型产生，在招募表达CCR7的免疫细胞（尤其是调节性T细胞，Treg cells）过程中发挥关键调控作用。CCL19/CCL21-CCR7轴参与HS发病进程的研究结果提示，其他表达CCR7的细胞也可能被招募并参与疾病进展。上述发现显著推进了我们对HS发病机制的认知，同时为未来针对CCR7的靶向治疗干预提供了极具潜力的研究方向。