Spectrophotometric Determination of Allura Red AC and Tartrazine in Food Products Using Hydrophobic Deep Eutectic Solvents as an Environmentally Sustainable Micro-Extractor

This paper reports the application of hydrophobic deep eutectic solvents (HDES) as an environmentally friendly alternative for liquid-phase microextraction (LPME) of Allura Red AC and Tartrazine in food products and the spectrophotometric determination of these analytes. The HDES used in this work were prepared by mixing octanoic acid (OctAc) or decanoic acid (DecAc) and tetrabutylammonium bromide (N4444-Br) in a molar ratio of 2:1. An investigation was also carried out regarding the effects of sample pH, sample volume, HDES volume, and concomitants on the analytical performance of the proposed method. The analytical curves obtained for Allura Red AC and Tartrazine in octanoic acid (OctAc)-tetrabutylammonium bromide (N4444-Br) were in the linear concentration ranges of 0.05 to 2.00 mg L-1 and 0.04 to 2.14 mg L-1, with limits of detection of 0.005 and 0.004 mg L-1, respectively. The proposed technique was successfully applied for the determination of Allura Red AC and Tartrazine in food products.本研究报道了疏水低共熔溶剂（hydrophobic deep eutectic solvents, HDES）作为环境友好型萃取剂，应用于食品中诱惑红AC与柠檬黄的液相微萃取（liquid-phase microextraction, LPME），并结合分光光度法对上述两种分析物进行定量检测。本研究所用HDES按2:1的摩尔比，将辛酸（octanoic acid, OctAc）或癸酸（decanoic acid, DecAc）与四丁基溴化铵（tetrabutylammonium bromide, N4444-Br）混合制备得到。此外，本研究考察了样品pH值、样品体积、HDES用量及共存物质对所提方法分析性能的影响。以辛酸-四丁基溴化铵为萃取溶剂时，诱惑红AC与柠檬黄的标准曲线线性浓度范围分别为0.05~2.00 mg·L⁻¹与0.04~2.14 mg·L⁻¹，检出限分别为0.005 mg·L⁻¹与0.004 mg·L⁻¹。所建立的分析技术已成功应用于食品中诱惑红AC与柠檬黄的定量检测。