HBc interactome analysis using MS-based quantitative proteomics.

ST-HBc-associated proteins were identified using MS-based proteomics. For this, cell lysate expressing ST-HBc or untagged HBc were treated or not with Benzonase before purification by affinity using Strep-Tactin. Eluted proteins were digested with trypsin and the resulting peptides submitted to MS-based proteomic analysis. The proteins were then identified and quantified in each sample before statistical analysis allowing to sort out proteins enriched with ST-HBc compared to untagged HBc used as negative control. (XLSX)