FIG1A- FIG1N.
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This excel table outlines the experimental setup for evaluating metabolic parameters in macrophages (MO) and those infected with Leishmania species (Leishmania amazonensis [LA] and Leishmania braziliensis [LB]). These results concern Fig 1A to 1N –Assessment of metabolic alterations in Leishmania-infected bone marrow-derived macrophages. FIG2A-FIG2B. MitoTracker Green columns A-C includes three groups: (A) Control group (Mock),(B) Leishmania amazonensis (La) infection,(C) Leishmania braziliensis (Lb) infection. MitoTracker Red columns E-G) shows the same groups: (E) Control group (Mock) (F) Leishmania amazonensis (La) infection (G) Leishmania braziliensis (Lb) infection. Five replicates were used for each experimental condition. These results concern Fig2A and Fig2B- Fig 2. Leishmania spp promote increased mitochondrial mass and membrane potential. FIG3A- FIG3B the results concern the infection rate and parasite load of macrophages infected with Leishmania amazonensis and Leishmania braziliensis. Evaluation of infection rate and parasite load in macrophages treated with metabolic inhibitors during L. amazonensis or L. braziliensis infections. Fig 4. Infection Index: concern the results shown in Fig 4 Infection index is calculated by multiplying the infection rate by the number of amastigotes. The values represent the average of parasite load and infection rate data.—Determination of infection index following metabolic inhibitor treatment. FIG 5. Results associated to the Fig 5 Inhibitor metabolic pathways did not affect binding and phagocytosis of the parasites Binding and phagocytosis in macrophages infected with Leishmania amazonensis and Leishmania braziliensis: FIG6B-Fig6F Results associated to the graphs shown in Fig 6 that evaluates mitochondrial reactive oxygen species (ROS) production: Effects of 2DG and Antimycin A treatment on ROS levels and Leishmania parasite load in infected macrophages. (XLSX)
本Excel表格概述了评估巨噬细胞（MO）以及感染利什曼原虫属（Leishmania species）物种——亚马逊利什曼原虫（Leishmania amazonensis [LA]）、巴西利什曼原虫（Leishmania braziliensis [LB]）——的巨噬细胞代谢参数的实验方案。 本结果对应图1A至1N——感染利什曼原虫的骨髓源性巨噬细胞（bone marrow-derived macrophages）代谢改变评估。 图2A~图2B：MitoTracker Green染色组（A~C列）设置三组样本：(A) 对照组（Mock）、(B) 亚马逊利什曼原虫（La）感染组、(C) 巴西利什曼原虫（Lb）感染组；MitoTracker Red染色组（E~G列）同样包含上述三组：(E) 对照组（Mock）、(F) 亚马逊利什曼原虫（La）感染组、(G) 巴西利什曼原虫（Lb）感染组。每组实验条件均设置5个生物学重复。 本结果对应图2A与图2B——图2：利什曼原虫可诱导线粒体质量与膜电位升高。 图3A~图3B的结果对应亚马逊利什曼原虫与巴西利什曼原虫感染巨噬细胞的感染率及寄生虫负荷，同时涵盖在亚马逊利什曼原虫或巴西利什曼原虫感染期间，经代谢抑制剂处理的巨噬细胞的感染率与寄生虫负荷评估。 图4：感染指数——本结果对应图4所示内容。感染指数通过感染率乘以无鞭毛体（amastigotes）数量计算得出，其数值为寄生虫负荷与感染率数据的平均值。——代谢抑制剂处理后感染指数的测定。 图5：本结果对应图5相关内容——代谢通路抑制剂不会影响寄生虫的结合与吞噬作用：亚马逊利什曼原虫与巴西利什曼原虫感染巨噬细胞的寄生虫结合与吞噬情况。 图6B~图6F：本结果对应图6所示的线粒体活性氧（reactive oxygen species, ROS）生成评估实验：2-脱氧葡萄糖（2DG）与抗霉素A（Antimycin A）处理对感染巨噬细胞内ROS水平及利什曼原虫寄生虫负荷的影响。(XLSX)
