Taurine Chloramine decreases cell viability and cytokine production in blood and spleen lymphocytes from septic rats

Abstract Taurine (Tau) is an abundant amino acid in polymorphonuclear leukocytes that react with hypochlorous acid to form taurine chloramine (TauCl) under inflammatory conditions. We investigated potential interactions between lymphocytes and TauCl in rats submitted to cecal ligation. Animals were divided into sham or CLP groups (24 or 120 h) to isolate lymphocytes from blood and spleen. Lymphocytes were cultured at a concentration of 1×106 cells/mL and activated by concanavalin A. Tau and TauCl were added at 1, 10, and 100 μM. Cells were incubated with MTT to evaluate cell viability and cytokine concentration in the supernatant was determined. TauCl decreased lymphocyte viability and altered the secretion pattern of important inflammatory mediators in non-specific-phenotype manner. The effort to a is elucidate mechanisms of immune cell (dys)function in sepsis is important to better understand the complex regulation of immune system during sepsis development, and further studies are necessary to confirm TauCl as potential target in this context.摘要 牛磺酸（Taurine，Tau）是多形核白细胞中含量丰富的氨基酸，在炎症状态下可与次氯酸反应生成牛磺酸氯胺（Taurine Chloramine，TauCl）。本研究探讨了接受盲肠结扎处理的大鼠体内淋巴细胞与TauCl之间的潜在相互作用。实验动物被分为假手术组与CLP组（分别于造模后24 h或120 h取材），从血液及脾脏中分离淋巴细胞。将淋巴细胞以1×10⁶ 个/毫升的浓度进行培养，并通过伴刀豆球蛋白A（Concanavalin A）激活；随后分别添加1、10及100 μM浓度的Tau与TauCl。采用MTT法孵育细胞以评估细胞活力，并测定上清液中的细胞因子浓度。结果显示，TauCl可降低淋巴细胞活力，并以非特异性表型方式改变重要炎症介质的分泌模式。阐明脓毒症中免疫细胞（功能异常）的调控机制，对于深入理解脓毒症发生发展过程中免疫系统的复杂调控网络具有重要意义，后续仍需开展进一步研究以验证TauCl是否可作为该疾病场景下的潜在治疗靶点。