Comparing the transcriptome expression differences between breast cancer cells EO771-tdT-Parental and EO771-tdT-LM2 using high-throughput sequencing.

The molecular mechanisms that regulate breast cancer cell (BCC) metastasis and proliferation within the leptomeninges (LM) are poorly understood, limiting development of effective therapies. Here we show that BCCs in mice can invade the LM by abluminal migration along blood vessels that connect vertebral/calvarial bone marrow and meninges, bypassing the blood-brain barrier. To screen for signaling pathways in EO-LM2 cells that could support their survival or proliferation in the meningeal niche, we performed RNA-seq analysis of EO771-tdT-Parental and EO771-tdT-LM2 to compare their transcriptome expression differences. Examination and comparision of the transcriptome expression in 2 cell types (EO771-tdT-Parental and EO771-tdT-LM2).调控乳腺癌细胞（breast cancer cell, BCC）在软脑膜（leptomeninges, LM）内转移与增殖的分子机制目前尚未得到充分阐明，这一现状限制了有效治疗策略的开发。本研究证实，小鼠体内的乳腺癌细胞可通过沿连接椎骨/颅盖骨骨髓与脑膜的血管进行腔外迁移，绕过血脑屏障侵袭软脑膜。为筛选出可支持EO-LM2细胞在脑膜微环境中存活或增殖的信号通路，我们对EO771-tdT-亲本株与EO771-tdT-LM2细胞开展了RNA测序（RNA-seq）分析，以比较二者的转录组表达差异。本研究对两种细胞类型（EO771-tdT-亲本株与EO771-tdT-LM2）的转录组表达情况进行了检测与对比分析。