Fig. 4Cells expressing His-Xpress tagged MIPS were grown in SC medium to the mid log phase and treated with 1 mM VPA for 3 h. MIPS activities were determined
Rumen samples from Bos indicus cattle were cultured using Rhodes grass (Chloris gayana) as a substrate in order to enrich for a consortium of lignocellulose degrading bacteria. DNA was extracted and u