transcriptome-wide map of NCSTN-KD and NC Hacat cells. transcriptome-wide map of NCSTN-KD and NC Hacat cells

Acne inversa (AI) is an inflammatory skin disease associated with the nicastrin (NCSTN) mutations. Family history with autosomal dominant inheritance has heen reported in AI patients and are associated with mutations in the γ-secretase subunit, nicastrin (NCSTN), presenilin enhancer 2 (PSENEN), and presenilin-1 (PSEN1). Among them, NCSTN gene has the highest mutation rate. To detect the impact of NCSTN deficiency on AI keratinocytes. Here, using the short hairpin RNA (shRNA)-mediated gene knockdown and RNA sequencing technology, we explored the differentially expressed genes regulated by NCSTN deficiency in HaCaT cells. Overall design: Examination of gene expression alternation in Hacat NC and NCSTN-KD cells反向痤疮（Acne inversa, AI）是一种与尼卡斯汀（nicastrin, NCSTN）突变相关的炎症性皮肤病。已有研究报道AI患者存在常染色体显性遗传的家族史，且此类病例与γ-分泌酶亚基尼卡斯汀（NCSTN）、早老素增强子2（presenilin enhancer 2, PSENEN）以及早老素-1（presenilin-1, PSEN1）的突变相关。其中，NCSTN基因的突变率最高。为探究NCSTN缺失对AI角质形成细胞的影响，本研究采用短发夹RNA（short hairpin RNA, shRNA）介导的基因敲低技术结合RNA测序技术，分析了HaCaT细胞中NCSTN缺失调控的差异表达基因。整体实验设计：检测HaCaT NC与NCSTN-KD细胞的基因表达变化。