Mean EC50 values and amplitude of responses for cAMP concentration-response curves in Figure 5.

EC50 values were determined for each individual experiment, using a sigmoidal dose-response equation. Compared to wild-type receptors, the EC50 values for N77Q-GIPR and N63,82,115Q-GLP-1R were significantly right shifted, as shown by a one-way ANOVA analysis followed by Dunnett's multiple comparison test. The changes in RFU values for the N62,77Q-GIPR were small and could not be accurately fitted. The amplitude of response was calculated by subtracting the lowest RFU values from the highest for each individual experiment.本研究采用S型剂量反应方程，对每项独立实验测定了半最大效应浓度（EC50）值。与野生型受体相比，N77Q-GIPR与N63,82,115Q-GLP-1R的EC50值显著右移，该结果经单因素方差分析（one-way ANOVA）并辅以Dunnett多重比较检验验证后得以确认。N62,77Q-GIPR的相对荧光单位（RFU）变化幅度较小，无法实现精准拟合。响应振幅通过对每项独立实验中最高RFU值与最低RFU值求差计算得到。