Expression data from mouse liver over-expressing either GFP alone or biotinylated-TRb1 and GFP

We over-expressed biotinylated-thyroid hormone receptor beta 1 (TRb1) in mouse liver using an adenovirus in order to perform ChIP-seq experiments. These microarrays were performed to determine gene expression changes in response to tri-iodothyronine (thyroid hormone; T3) stimulation. A control GFP adenovirus was used and gene expression from these livers was also done as a comparison. We performed microarrays from Ad-GFP-infected propylthiouracil (PTU)-fed livers injected with either saline or T3 and Ad-TRb1-GFP infected livers injected with either saline or T3. RNA was extracted from livers of biotin ligase (BirA)-expressing mice that had been infected with either Ad-GFP or Ad-TRb1, fed with PTU for 3 weeks followed by saline or T3 injections for 4 consecutive days.为开展染色质免疫沉淀测序（ChIP-seq）实验，我们通过腺病毒载体在小鼠肝脏中过表达生物素化甲状腺激素受体β1（biotinylated-thyroid hormone receptor beta 1，TRb1）。本研究开展基因芯片实验，以探究三碘甲状腺原氨酸（tri-iodothyronine，甲状腺激素；T3）刺激诱导的基因表达变化。本研究同时设置携带绿色荧光蛋白（Green Fluorescent Protein，GFP）的对照腺病毒感染组，以该组肝脏样本的基因表达数据作为对照。我们对以下四组样本开展基因芯片检测：经丙基硫氧嘧啶（propylthiouracil，PTU）喂养并分别注射生理盐水或T3的Ad-GFP感染小鼠肝脏样本，以及经Ad-TRb1-GFP感染并分别注射生理盐水或T3的小鼠肝脏样本。本研究提取的RNA来自表达生物素连接酶（BirA）的小鼠肝脏，这些小鼠分别感染Ad-GFP或Ad-TRb1，经PTU喂养3周后，连续4天注射生理盐水或T3。