Coastal marshes (Louisiana) Targeted Locus (Loci)

This study was done to evaluate changes in indigenous microbial communities from coastal marsh sediments after the Gulf of Mexico oil spill in 2010. This study was conducted from eleven coastal marshes in southern Louisiana. Samples were acquired from May 2010 through August 2013, focusing on sediments approximately 1 m offshore from the edge of the marsh. Marshes were oiled from late June-August 2010, and some marshes were reoiled in 2011 from tropical storms and hurricanes. Some marshes close to the Mississippi River were impacted by freshwater diversions in 2010. Bacterial communities were assessed from sediments at 0-1 cm and 1-2 cm depth intervals. The results are amplicon pyrosequence libraries of single-direction reads generated using multiplexed, bar-coded primers targeting the V1-V3 region of the bacterial of 16S rRNA gene.本研究旨在评估2010年墨西哥湾溢油事件后，沿海沼泽沉积物中土著微生物群落的变化情况。本研究选取路易斯安那州南部的11处沿海沼泽作为研究样地，采样周期为2010年5月至2013年8月，采样点位聚焦于距沼泽岸线约1米处的沉积物。这些沼泽于2010年6月下旬至8月间遭受原油污染，部分沼泽还因2011年的热带风暴与飓风发生了二次溢油。部分毗邻密西西比河的沼泽在2010年受到淡水分流工程的影响。研究人员分别采集0-1厘米及1-2厘米深度层的沉积物样本以解析细菌群落组成，所得结果为采用多重条形码引物靶向扩增细菌16S rRNA基因V1-V3高变区所构建的扩增子焦磷酸测序文库，包含单向测序读段。