What MiRNAs Are Expressed In The E11.5 Mouse Midfacial Tissue?

Researching what miRNAs are expressed in the E11.5 mouse midfacial tissue. Methods: miRNA extracted using the Norgen miRNA Purification Kit, miRNA libraries were prepared using BiooScientific NEXTflex Small RNA Sequencing Kit by ligation of 3' barcoded adapters and 5' adapters on small-RNA sequences; miRNA-seq Illumina HiSeq 2000.本研究旨在探究胚胎发育第11.5天（E11.5）小鼠面中部组织中表达的微小RNA（microRNA，miRNA）。实验方法如下：采用Norgen miRNA纯化试剂盒提取miRNA；使用BiooScientific NEXTflex小RNA测序试剂盒，通过在小RNA序列上连接3'端带条形码的接头与5'端接头的流程构建miRNA文库；随后利用Illumina HiSeq 2000平台完成miRNA测序（miRNA-seq）。