Malat1 regulates intestinal epithelial cell function

The long-non-coding RNA Malat1 is abundantly expressed in all subsets of the intestinal epithelial cells (IEC). Here, we compared the transcriptome of the small intestinal and colonic epithelium of wildtype and Malat1-/- mice under steady state. We found that Malat1 is involved in IEC homeostasis, intestinal microbial balance, and regulates programs involved in intestinal cancer. Overall design: Two intestine tissues from each genotype were obtained from 9-11 week old cohoused littermate. Results of epithelium from Malat1-deficient (Malat1-/-) mice were compared to those from their gender-matched controls (Malat1+/+ or Malat1+/-).长链非编码RNA Malat1在肠道上皮细胞（intestinal epithelial cells，IEC）的所有亚群中均呈高丰度表达。本研究对稳态条件下野生型与Malat1基因敲除（Malat1-/-）小鼠的小肠及结肠上皮转录组开展了比较分析。研究发现，Malat1参与肠道上皮细胞稳态维持与肠道微生物群落平衡，并调控与肠道癌变相关的基因表达程序。总体实验设计：从9至11周龄同笼饲养的同窝小鼠中，每种基因型获取两份肠道组织样本；将Malat1缺陷型（Malat1-/-）小鼠的上皮细胞转录组结果，与其性别匹配的对照小鼠（Malat1+/+或Malat1+/-）的结果进行比对。