MicroRNA expression during Hematopoiesis

Murine ES cells were grown on confluent OP-9 and differentiated along the hematopoitic lineage. To assess the effects of Notch in hematopoiesis, activated Notch was overexpressed from Day 5 to Day 8. Samples included Day 5 (control) and Day 8 Notch On and Day 8 Notch Off, the samples were done in triplicates.小鼠胚胎干细胞（Murine ES Cells）在汇合状态的OP9饲养层细胞上培养，并沿造血谱系进行分化。为评估Notch在造血过程中的作用，研究人员于第5天至第8天过表达激活型Notch蛋白。实验样本包括第5天对照组、第8天激活型Notch开启组及第8天激活型Notch关闭组，所有样本均设置三次生物学重复。