Figure3_Raw CT data

<b>Figure 4. mRNA for four of predicted GOI are altered in human sAD2.1 iPSNs compared to control AX0018 iPSNs. a-b. </b>Quantitative immunocytochemistry showing significant increase of pS199/pS202 (AT8) tau in βIII-tubulin (neuronal marker) positive sAD2.1 iPSNs compared to control AX0018 iPSNs. <b>c-d. </b>Western blot and quantification showing significantly increased levels of total, pS199/pS202 (AT8), and pT231 (AT180) positive tau levels in sAD2.1 iPSNs compared to control AX0018 iPSNs. <b>e. </b>Quantitative real-time PCR analysis showing two folds increase in the mRNA levels of RAB40A, SCGB3A1, TMEFF2, and a fold decrease in FRRS1 in sAD2.1 iPSNs compared to AX0018 control iPSNs. Data shown are mean + s.e.m; Two-tailed <i>t </i>tests welch-corrected; *p&lt;0.05; **p&lt;0.01, ***p&lt;0.005, n=3 biological replicates; n=3 technical replicates.**图4.** 4个预测目的基因（GOI，Gene of Interest）的mRNA在人类sAD2.1诱导多能干细胞衍生神经元（iPSNs，induced pluripotent stem cell-derived neurons）中的表达水平，与对照AX0018诱导多能干细胞衍生神经元相比发生改变。a-b部分：定量免疫细胞化学染色结果显示，在βIII微管蛋白（βIII-tubulin，神经元标志物）阳性的sAD2.1 iPSNs中，pS199/pS202位点磷酸化tau蛋白（AT8）的表达量显著高于对照AX0018 iPSNs。c-d部分：蛋白质印迹（Western blot）实验及定量分析结果显示，与对照AX0018 iPSNs相比，sAD2.1 iPSNs中总tau蛋白、pS199/pS202位点磷酸化tau蛋白（AT8）以及pT231位点磷酸化tau蛋白（AT180）的表达水平均显著升高。e部分：实时定量PCR分析结果显示，相较于AX0018对照iPSNs，sAD2.1 iPSNs中RAB40A、SCGB3A1、TMEFF2的mRNA水平上调2倍，而FRRS1的mRNA水平下调1倍。本实验数据以均值±标准误（s.e.m.）呈现；采用双侧韦尔奇校正t检验进行统计学分析；*p<0.05，**p<0.01，***p<0.005；生物学重复数n=3，技术重复数n=3。