The identification of miRNA targets by Ago2-CLIP methods has provided major insights into the biology of this important class of non-coding RNAs. However, these methods are technically challenging and
We generated Gadd45a,b,g triple-knockout mouse embryonic stem cells and performed RNA-seq expression profiling under six different conditions of cell culture and in vitro differentiation. Overall desi
We identified Letm1 domain containing 1 (Letmd1) as a candidate regulator of BAT thermogenesis. We generated Letmd1 KO mice, raised at thermoneutral or cold conditions, and performed RNA-sequencing in