Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptomes in fetal and 2 weeks old rat islets

Unlike adult ß-cells, fetal and neonatal islets are functional immature and have blunted glucose responsiveness and decreased insulin secretion in response to stimuli. Pancreatic islets are a mixture of different cell types. Study of transcriptomes from intact islets would identify novel molecular mechanisms controlling islet functional development. At e19 and 2 weeks of age, pancreatic islets were isolated and total RNA were extracted for RNA-Seq study. Gene expression profiles were compared in the study. Overall design: Examination of transcriptomes in 2 time points (e19 and 2 weeks of age)与成年β细胞不同，胎儿期与新生期的胰岛处于功能未成熟状态，其葡萄糖反应性减弱，且在受到刺激时胰岛素分泌水平降低。 胰腺胰岛是多种细胞类型的混合体。 对完整胰岛的转录组（transcriptomes）进行研究，可揭示调控胰岛功能发育的全新分子机制。 于胚胎19天（e19）及出生后2周龄时分离胰腺胰岛，提取总RNA以开展RNA测序（RNA-Seq）研究。 本研究对基因表达谱进行了比较分析。 整体实验设计：对两个时间点（胚胎19天及出生后2周龄）的胰岛转录组进行检测分析。