Loss of real-time PCR signal (ΔCt value) from viable bacteria by staining with EMA or PMA.

106 viable C. jejuni cells were spiked into 1 ml of either phosphate buffered saline (PBS), buffered peptone water (BPW) or chicken rinse (cRinse). Staining was performed for 5 or 15 min in the dark at room temperature. Mean ΔCt values (with and without dye) ± standard deviation are shown.ex.phase +24 h cells, bacteria grown for 18–24 h on Columbia blood agar or exponentially in liquid medium; 48 h stat. cells, bacteria grown for 40–48 h on Columbia blood agar; all, viable bacteria without differentiation of the growth phase; n. d., not determined; n, number of tested samples.将106株活菌空肠弯曲杆菌（C. jejuni）接种至1 mL磷酸盐缓冲液（PBS）、缓冲蛋白胨水（BPW）或鸡肉冲洗液（cRinse）中。染色过程于室温、避光条件下进行5或15分钟。本文展示了添加染料与未添加染料条件下的平均ΔCt（循环阈值差值）值±标准差。其中，ex. phase +24 h cells指在哥伦比亚血琼脂平板上培养18~24 h，或于液体培养基中进行指数生长期培养的细菌；48 h stat. cells指在哥伦比亚血琼脂平板上培养40~48 h的细菌；all代表未区分生长阶段的活菌；n. d.代表未测定；n代表受试样本数量。