Additional file 6 of Exploring RNA cargo in extracellular vesicles for pleural mesothelioma detection

Additional file 6: Table S1. Mapping rate of RNA-seq reads from PM-EV samples. Mapped reads were obtained with STAR. Table S2. Biotypes of genes expressed among 4 PM-EV samples. Expressed genes are those with Kallisto counts > 0 in all 4 samples. Table S3. TPM expression of the genes expressed across 4 PM-EV samples. TPM values provided by Kallisto. Table S4. Qsmooth batch-corrected and TMM normalized counts of Plasma-EV and PM-EV samples. Table S5. EdgeR results of differential gene expression between PM-EV and Plasma-EV samples. S and E marker genes are reported in the “marker” column. Table S6. Results of qPCR analysis of selected genes in PM-EVs and matching primary cells. Table S7. Results of fgsea enrichment analysis in PM-EV samples. Table S8. Sequences of primers used for qPCR analysis. Table S9. Characteristics of healthy plasma donors from GSE100206 dataset. Table S10. Clinical characteristics associated with the non-PM cells used for qPCR analysis. Table S11. Results of qPCR analysis of selected genes in PM-EVs and non-PM-EVs.补充文件6：表S1。PM-EV样本的RNA测序（RNA-seq）读段比对率。比对得到的测序读段通过STAR软件获取。 表S2。4个PM-EV样本中表达基因的基因生物型。表达基因定义为在全部4个样本中Kallisto计数均大于0的基因。 表S3。4个PM-EV样本中所有表达基因的每百万转录本（Transcripts Per Million，TPM）表达量。TPM值由Kallisto软件计算得到。 表S4。血浆细胞外囊泡（Plasma-EV）与PM-EV样本的Qsmooth批次校正及TMM标准化计数。 表S5。PM-EV与血浆细胞外囊泡样本间的差异基因表达EdgeR分析结果。"marker"列中报告了S与E标记基因。 表S6。PM-EVs及匹配原代细胞中选定基因的定量聚合酶链式反应（qPCR）分析结果。 表S7。PM-EV样本中的fgsea富集分析结果。 表S8。用于qPCR分析的引物序列。 表S9。来自GSE100206数据集的健康血浆捐献者特征信息。 表S10。用于qPCR分析的非PM细胞相关临床特征。 表S11。PM-EVs与非PM-EVs中选定基因的qPCR分析结果。