Phylogenetic analysis of the MADS-box genes differentially expressed among different floral organs.

Amino acid sequences were aligned by the ClustalW 2.0, and phylogenetic relationships were reconstructed using a maximum-likelihood (ML) method in PHYML software with JTT amino acid substitution model. Previously published plant MADS-box protein sequences were retrieved from GenBank database (PeMADS2: AAR26628, CeAP3-like1: AFH66788, CeAP3-like2: AFH66787, CgAP3:ADI58460, CeMADS2: ADP00516, CeMADS1: ADP00515, CePI-like: AFH66786, OMADS11: ADJ6724, OMADS6: ADJ67238, OMADS1: ADJ67237, OMADS10: ADJ67240, OMADS2: AIJ29175, OMADS3: AAO45824, OMADS4: AIJ29176, DthyrAG1: AAY86364, DthyrAG2: AAY86365, PhalAG1: BAE80120, PhalAG2: BAE80121, PeMADS2: AY378149, PeMADS3: AY378150, PeMADS4: AY378151, PeMADS5: AY378148).采用ClustalW 2.0对氨基酸序列进行比对；随后借助PHYML软件，以JTT氨基酸替换模型为基础，通过最大似然（maximum-likelihood，ML）法重建系统发育关系。此前已发表的植物MADS-box蛋白序列均从GenBank数据库中检索获取，对应的序列登录号如下：PeMADS2: AAR26628、CeAP3-like1: AFH66788、CeAP3-like2: AFH66787、CgAP3:ADI58460、CeMADS2: ADP00516、CeMADS1: ADP00515、CePI-like: AFH66786、OMADS11: ADJ6724、OMADS6: ADJ67238、OMADS1: ADJ67237、OMADS10: ADJ67240、OMADS2: AIJ29175、OMADS3: AAO45824、OMADS4: AIJ29176、DthyrAG1: AAY86364、DthyrAG2: AAY86365、PhalAG1: BAE80120、PhalAG2: BAE80121、PeMADS2: AY378149、PeMADS3: AY378150、PeMADS4: AY378151、PeMADS5: AY378148。