Dyadic encounter unfamiliar female pairs over 2 days – 16p11.2 Del mouse strain – Del/+-Del/+ - 5170_5256

We evaluated the social interactions and communication between unfamiliar individuals in pairs. For these recordings of social behaviour and ultrasonic communication, we focused on pairs of individuals since we currently cannot identify the emitter of USVs when animals were interacting closely. Therefore, we recorded undisturbed dyadic interactions between two unfamiliar individuals (from two different housing cages) of the same age (14-20 weeks of age) and genotype for 47h (two days and nights). For that purpose, we coupled the Live Mouse Tracker system (plugin 931) with one CM16/CMPA microphone (Avisoft Bioacoustics, Glienecke, Germany) connected to the Avisoft Ultrasound Gate 416 (300 kHz sampling rate, 16-bit format; trigger: level of this channel; pre-trigger: 1 s; hold time: 1 s; duration &gt; 0.005 s; trigger event: 2 % energy in 25-125 kHz with entropy &lt; 50%; Avisoft Bioacoustics, Glienecke, Germany). LMT and Avisoft systems were synchronised based on the protocol described in (de Chaumont et al., 2021). Each upload includes the sqlite database from LMT (processed, with USV synchronised) and a zip file with all USVs sequences automatically recorded by Avisoft.本研究评估了成对陌生个体间的社会互动与交流行为。针对此类社会行为与超声通讯的录音工作，我们将研究对象限定为成对个体，原因在于当前无法在动物近距离互动时分辨超声发声（ultrasonic vocalizations, USVs）的发声个体。因此，我们对来自不同饲养笼、年龄为14至20周龄且基因型一致的两只陌生个体，开展了为期47小时（两个昼夜）的无干扰成对互动录制。为此，我们将活体小鼠追踪系统（Live Mouse Tracker system，插件编号931）与一支CM16/CMPA麦克风（德国格利尼克市Avisoft Bioacoustics公司生产）相连，该麦克风再接入Avisoft Ultrasound Gate 416超声采集设备，其参数设置如下：采样率300 kHz，16位格式；触发条件：该通道电平；预触发时长1 s；保持时长1 s；有效录制时长>0.005 s；触发事件：25~125 kHz频段内能量占比2%且熵值<50%；该设备亦由德国格利尼克市Avisoft Bioacoustics公司生产。活体小鼠追踪系统（LMT）与Avisoft采集系统基于de Chaumont等人2021年发表的实验方案实现了时间同步。每份上传文件均包含来自LMT的SQLite数据库（已完成数据处理并完成USVs同步），以及一份收录了Avisoft设备自动录制的全部USVs序列的压缩归档文件。