Sox4 null mice e12.5 pancreas

The purpose of this experiment was to assess global changes in gene expression pattern in the pancreas from Sox4-/- embryos vs. pancreas from Sox4 wild-type embryos at embryonic day 12.5. [Sox4 gene knock-out (Schilham, M. W. et al. (1996) Defects in cardiac outflow tract formation and pro-B-lymphocyte expansion in mice lacking Sox-4. Nature 380, 711-4.)] Embryonic pancreases from homozygous mutants and their wild-type littermates were harvested. Total RNA was isolated amplified using a linear amplification kit and used to prepare the hybridization samples through indirect labeling. Keywords = Pancreas Sox4 null e12.5 Keywords: parallel sample本实验旨在评估胚胎发育第12.5天时，Sox4基因敲除（Sox4-/-）小鼠胚胎胰腺与Sox4野生型小鼠胚胎胰腺的基因表达模式全局变化。 [Sox4基因敲除模型引自Schilham M.W.等人1996年的研究：缺失Sox-4的小鼠可出现心脏流出道形成缺陷与前B淋巴细胞扩增异常，《自然》，380卷，711-714页] 收集纯合突变型小鼠及其野生型同窝仔鼠的胚胎胰腺，提取总RNA后采用线性扩增试剂盒进行扩增，通过间接标记法制备杂交样本。 关键词：胰腺、Sox4敲除、胚胎发育第12.5天、平行样本。