Primers used to amplify recombinant wild- type and attenuated <i>spEX</i> from <i>Staphylococcus pseudintermedius</i> and pUC19-<i>spEX-</i>M plasmid.

NotI and BamHI restriction sites are bold and underlined, nucleotide sequences belonging to SpEX and SpEX-M are show in italics, the primer binding sites are in brackets and sequences outside SpEX and SpEX-M (GCATGA) were used to flank NotI and BamHI recognition sites.NotI与BamHI限制性酶切位点以加粗并带下划线的形式标注；属于SpEX与SpEX-M的核苷酸序列以斜体显示；引物结合位点置于括号中；位于SpEX与SpEX-M外侧的GCATGA序列，被用作NotI与BamHI识别位点的侧翼序列。