Accumulation of HT-2 toxin from contaminated mushroom compost by edible Agaricus bisporus

Wheat straw is commonly used as a cellulose source in mushroom compost and could be a secondary source of mycotoxin contamination in the food chain. We cultivated edible Agaricus bisporus and Pleurotus ostreatus on T-2/HT-2 artificially-contaminated mushroom compost and developed and in-house validated an UHPLC-MS/MS method for determination of T-2, HT-2, T2-triol and T2-tetraol in mushroom compost and mushroom basidiocarp. A rapid phase I metabolization of T-2 and HT-2 in mushroom compost was observed. In Agaricus bisporus, basidiocarps 8–15 µg kg−1 accumulation of HT-2 calculated on wet weight was measured. No detectable mycotoxins were found in Pleurotus ostreatus basidiocarp.麦秆常被用作菌菇栽培基质的纤维素来源，同时也可能成为食物链中真菌毒素污染的次级来源。本研究将可食用的双孢蘑菇（Agaricus bisporus）和平菇（Pleurotus ostreatus）接种于经T-2/HT-2人工污染的菌菇栽培基质中，建立并经实验室内部验证了一种超高效液相色谱-串联质谱法（UHPLC-MS/MS）检测方法，用于测定菌菇栽培基质及菌菇子实体中的T-2、HT-2、T2-三醇（T2-triol）与T2-四醇（T2-tetraol）含量。研究观察到T-2与HT-2在菌菇栽培基质中发生了快速的I相代谢。在双孢蘑菇子实体中，测得以湿重计的HT-2积累量为8~15 μg·kg⁻¹。平菇子实体中未检出任何可检测水平的真菌毒素。