Insights into Evolutionary divergence of Piwi genes in Ae. albopictus.

A) McDonald-Kreitman test for each Piwi gene using the orthologous sequences of Ae. aegypti as outgroup. NI = Neutrality Index; Alpha = proportion of base substitutions fixed by natural selection; P estimated using Fisher’s exact test. B) Output of Codeml with significant results regarding sites under positive selection.A) 以埃及伊蚊（Ae. aegypti）的直系同源序列（orthologous sequences）作为外类群（outgroup），针对每个Piwi基因开展麦克唐纳-克里特曼检验（McDonald-Kreitman test）。其中NI为中性指数（Neutrality Index）；Alpha代表经自然选择固定的碱基替换比例；P值通过费希尔精确检验（Fisher’s exact test）估算得出。 B) Codeml的分析输出结果，其中包含与正选择作用位点相关的显著统计结果。