Comparison of the kinetic parameters of the isocitrate oxidation activity of the wild-type and mutant IDH enzymes.

aWhen calculating the Km for isocitrate, the concentration of D-isocitrate was calculated as 50% of the total DL-isocitrate in this study. “na” indicates no measurable activity.Comparison of the kinetic parameters of the isocitrate oxidation activity of the wild-type and mutant IDH enzymes.本研究中，计算异柠檬酸（isocitrate）的米氏常数（Km）时，将D-异柠檬酸（D-isocitrate）的浓度计为总DL-异柠檬酸（DL-isocitrate）的50%。"na"代表未检测到可测活性。野生型与突变型异柠檬酸脱氢酶（isocitrate dehydrogenase, IDH）的异柠檬酸氧化活性动力学参数比较。