Next Generation Sequencing Quantitative Analysis of Wild Type and TMEM9 KD Transcriptomes

Purpose: The goals of this study are to check signaling pathway change upon TMEM9 KD using NGS-derived retinal transcriptome profiling (RNA-seq). Methods: Retinal mRNA profiles of MDA-MB-453 WT and TMEM9 KD were generated by deep sequencing, using Illumina. The sequence reads that passed quality filters were analyzed. Results: Using GSEA analysis, we found mTOR signaling pathway was suppressed after TMEM9 KD. Conclusions: Our study revealed the signaling pathway change upon TMEM9 KD in BRCA cells. Overall design: Retinal mRNA profiles of MDA-MB-453 cells研究目的：本研究旨在利用下一代测序（NGS）来源的视网膜转录组表达谱（RNA-seq）数据，检测TMEM9基因敲低（KD）后细胞信号通路的变化情况。 研究方法：采用Illumina测序平台对MDA-MB-453野生型（WT）与TMEM9敲低（KD）细胞的视网膜mRNA进行深度测序，获取转录组数据；对通过质量过滤的序列读段开展后续分析。 研究结果：通过基因集富集分析（GSEA）发现，TMEM9基因敲低后，mTOR信号通路受到抑制。 研究结论：本研究揭示了BRCA细胞中TMEM9基因敲低后的信号通路变化情况。 整体实验设计：MDA-MB-453细胞的视网膜mRNA转录组表达谱。