Genome mining for ribosomally synthesized and post-translationally modified peptides (RiPPs) in <i>Streptomyces</i> bacteria

Ribosomally synthesized post-translationally modified peptides (RiPPs) are a novel category of bioactive natural products (NPs). Streptomyces bacteria are a potential source of many bioactive NPs. Limited opportunities are available to characterize all the bioactive NP gene clusters. In this study, 410 sequences of Streptomyces were analyzed for RiPPs through genome mining using the National Center for Biotechnology Information (NCBI), by combining BAGEL and anti-SMASH. A total of 4098 RiPPs were found; including both classified (lanthipeptide, RiPP-like, bacteriocin, LAPs, lassopeptide, thiopeptides) and nonclassified RiPPs. Soil was identified as a rich habitat for RiPPs. These data may offer alternative future remedies for various health issues.核糖体合成并经翻译后修饰的肽（Ribosomally synthesized post-translationally modified peptides, RiPPs）是一类新兴的生物活性天然产物（bioactive natural products, NPs）。链霉菌属细菌是众多生物活性天然产物的潜在来源。当前对全部生物活性天然产物基因簇的功能解析仍存在较大局限。本研究依托美国国家生物技术信息中心（National Center for Biotechnology Information, NCBI）的数据库，结合BAGEL与anti-SMASH工具开展基因组挖掘分析，对410条链霉菌序列中的RiPPs进行了系统筛查。最终共检索得到4098条RiPPs，涵盖已分类的羊毛硫肽、类RiPP肽、细菌素、LAPs、套索肽、硫肽类，以及未分类的RiPPs。研究发现土壤是RiPPs的丰富生存栖息地。本研究所得数据可为未来解决各类健康问题提供新的备选治疗方案。