OWRC Carbon and HABs

In this Ohio Water Resources Council funded collaboartion between the labs of Rachel Gabor at Ohio State University and Rachel Eveleth at Oberlin College, the seasonal succession of inorganic and organic carbon and their drivers in the Western Basin of Lake Erie was assessed. Three surveys from the mouth of the Maumee River to the Bass Islands were completed in June (R/V Erie Monitor), August (R/V Erie Monitor) and October (R/V Gibraltar III) of 2022. Particulate and dissolved organic carbon, alkalinity, pH, and dissolved inorganic carbon were measured along with temperature, conductivity, chlorophyll, oxygen, nutrients, fluorescence and Microcystin. All sample bottles triple rinsed and stored under appropriate conditions for analysis. Alkalinity- pH probe calibration was performed daily and standards run every 25 samples were within 5% of expected. TOC/DOC- a 6 point calbration curve and run check standards (high and low concentration) every 8 samples and blanks are run throughout. YSI- oxgyen and pH calibration completed prior to each use. Fluorescence and UV-Vis- standard instrument checks before each set of samples and then applied instrument corrections, raman-noramlize and blank subtract. FluoroProbe- recorded and averaged five stable measurements and data was normalized by the measured Chl-a concentrations. Nutrients- All analyzer runs (dissolved and total) had 8 non-zero standards and a zero concentration standard, and runs were only accepted if the R2 was >0.999.本数据集由俄亥俄州水资源委员会资助，为俄亥俄州立大学Rachel Gabor实验室与奥柏林学院Rachel Eveleth实验室的合作研究成果，针对伊利湖西部盆地无机碳与有机碳的季节演替及其驱动因子开展了系统评估。 2022年6月（使用R/V Erie Monitor科考船）、8月（R/V Erie Monitor）及10月（R/V Gibraltar III）共完成3次从莫米河河口至巴斯群岛的野外调查。本次研究测定了颗粒态有机碳、溶解态有机碳、碱度、pH值与溶解态无机碳，同时同步记录了水温、电导率、叶绿素浓度、溶解氧、营养盐含量、荧光信号以及微囊藻毒素（Microcystin）水平。 所有样品瓶均经三次润洗，并在符合分析要求的条件下储存。各检测项目的校准与质控标准如下： 1. 碱度-pH探头每日进行校准，每25个样品插入标准品进行质控，其检测结果与理论预期值的偏差需控制在5%以内； 2. 总有机碳/溶解有机碳（TOC/DOC）分析采用6点校准曲线，每8个样品插入高低浓度质控标准品进行核查，全程同步设置空白对照； 3. YSI多参数分析仪：每次使用前完成溶解氧与pH校准； 4. 荧光与紫外-可见光谱分析：每组样品测试前均开展标准仪器校验，随后通过拉曼归一化与空白扣除法完成仪器校正； 5. 荧光探头（FluoroProbe）：记录并取5次稳定测量值的平均值，检测数据以实测叶绿素a浓度进行归一化处理； 6. 营养盐分析：所有分析仪测试（涵盖溶解态与总态组分）均设置8个非零浓度标准品与1个零浓度标准品，仅当拟合决定系数R²>0.999时，该批次测试结果方可被认可。