Putative membrane proteins identified in <i>T. vaginalis</i> hydrogenosomes (continued).

Proteins were manually annotated based on searches in TrichDB, Uniprot, and PFAM A+B (Table S2). Protein structure was predicted using TMHMM and MEMSAT3; subcellular location was predicted using TargetP and PsortII. TM No., number of predicted transmembrane α-helixes. M, predicted location in mitochondria. S - predicted proteins of secretory pathway; O, predicted location in other compartments; Mit%, probability percentage of mitochondrial location; *, mitochondrial location was not predicted; Exp. Local., experimental location; H, localization of HA-tagged proteins was confirmed in T. vaginalis hydrogenosomes by immunofluorescence microscopy; NV, transformed T. vaginalis strain was not viable. Signal: Δ indicates N-terminal targeting sequence identified by Hunter; β indicates presence of beta signal of beta-barrel proteins for insertion into the outer membrane of mitochondria; Ct, C-tail anchor detected [Fig. S2].本数据集所涉及的蛋白质均基于TrichDB（毛滴虫数据库）、UniProt（通用蛋白质数据库）及PFAM A+B（蛋白质家族数据库A+B）的检索结果完成人工注释（详见补充表S2）。蛋白质结构采用TMHMM（跨膜螺旋预测工具）与MEMSAT3（膜蛋白结构预测算法）进行预测，亚细胞定位则通过TargetP（亚细胞定位预测工具）与PsortII（亚细胞定位预测软件）完成预测。各标记含义如下：TM No.指预测得到的跨膜α螺旋数量；M代表预测定位于线粒体；S代表预测为分泌通路相关蛋白质；O代表预测定位于其他细胞器区室；Mit%为线粒体定位概率百分比；*表示未预测到线粒体定位；Exp. Local.为实验验证的亚细胞定位；H表示通过免疫荧光显微镜证实HA标签标记蛋白在阴道毛滴虫氢化酶体中的定位；NV表示转化后的阴道毛滴虫菌株无法存活。信号标记说明如下：Δ代表经Hunter鉴定得到的N端靶向序列；β代表β桶状蛋白存在可介导其插入线粒体外膜的β信号序列；Ct表示检测到C端锚定序列（详见补充图S2）。