Annual dynamics and metagenomics of marine vesicles: a layer of complexity in the dissolved organic fraction

The data consists of Illumina raw reads obtained from prokaryotes from coastal Mediterranean seawaters in Alicante, Spain, over the course of one year. Illumina DNA prep kit (IDT, Ref. 20026930) was used following the manufacturer's protocol to generate and sequence the libraries for the obtained DNA samples from prokaryotic metagenomes. Subsequently, these libraries were sequenced using HiSeq X technology (150 PE, 1 lane) by two different facilities: Macrogen in Seoul, Republic of Korea, and the Genomics Unit of the Center for Genomic Regulation (CRG) in Barcelona, Spain. The dataset was used in order to ascertain the main microbial producers involved in the release of DNA packaged in EVs in our annual sampling. The samples are named according to the convention 'prokmeta[Month], where 'prokmeta' signifies prokaryotic metagenome, '[Month]' indicates the month of sampling, and "1" represents the forward , while (2) represent the reverse read. For instance, 'prokmetaDEC.1' denotes the prokaryotic metagenome for sample of December, with '1' indicating the forward read, while 'prokmetaDEC.2' represents the corresponding reverse read. This naming scheme is consistent across all samples in the dataset from sample of November 2021 to sample of June 2022.本数据集包含从西班牙阿利坎特地中海沿岸海水中的原核生物中获取的Illumina原始测序读段，采样周期为一年。实验采用Illumina DNA制备试剂盒（IDT，货号20026930），严格遵循制造商的操作流程，为原核宏基因组DNA样本构建测序文库并完成测序。随后，这批文库由两家不同的测序机构使用HiSeq X技术（150 bp双端测序，1个泳道）完成测序：分别为韩国首尔的Macrogen公司，以及西班牙巴塞罗那基因组调控中心（CRG）的基因组学单元。本数据集旨在明确本次年度采样中，参与释放包裹于细胞外囊泡（Extracellular Vesicles, EVs）内DNA的主要微生物类群。 所有样本均遵循"prokmeta[Month]"的命名规范：其中"prokmeta"代表原核宏基因组（prokaryotic metagenome），"[Month]"为采样月份，"1"表示正向测序读段，"2"则表示反向测序读段。例如，"prokmetaDEC.1"代表12月采样的原核宏基因组样本，其中"1"对应正向测序读段，"prokmetaDEC.2"则为其对应的反向测序读段。本数据集的全部样本均采用此命名规则，采样时间范围为2021年11月至2022年6月。