Next-Generation Sequencing Facilitates Quantitative Analysis of Wild Type and DDX5 knockdown of GC Transcriptomes

We aimed to detect the mRNA expression levels in HGC-27 cells after transduction with lentivirus harboring DDX5 shRNA or non-targeting shRNA. Overall design: Totally, two samples were examined: HGC-27 cell transduced with lentivirus harboring DDX5 shRNA or non-targeting shRNA.本研究旨在检测转导携带DDX5短发夹RNA（short hairpin RNA）或非靶向短发夹RNA（short hairpin RNA）的慢病毒后，HGC-27细胞的mRNA表达水平。整体实验设计：共检测2组样本，一组为转导携带DDX5 shRNA的慢病毒的HGC-27细胞，另一组为转导携带非靶向shRNA的慢病毒的HGC-27细胞。