Single Cell and Spatial Analysis of Human Squamous Cell Carcinoma [CRISPR screen]

To define the cellular composition and spatial architecture of the tumor micoenvironment, we combined single-cell RNA-sequencing, spatial transcriptomics, and multiplexed ion-beam imaging from 10 patient cutaneous squamous cell carcinoma tumors and site-matched normal skin SCC cell lines were infected with a CRISPR sgRNA library of 334 genes (and 136 non-targeting sgRNA controls) and grown either as xenograft tumors in immunocomprised mice or in vitro, followed by sequencing of sgRNAs为解析肿瘤微环境的细胞组成与空间结构，我们整合了来自10例皮肤鳞状细胞癌（cutaneous squamous cell carcinoma）患者肿瘤及位点匹配正常皮肤样本的单细胞RNA测序（single-cell RNA-sequencing）、空间转录组学（spatial transcriptomics）与多重离子束成像（multiplexed ion-beam imaging）数据。同时，我们将鳞状细胞癌细胞系感染含334个基因的CRISPR单引导RNA（sgRNA）文库（配套136个非靶向sgRNA对照），分别于免疫缺陷小鼠体内构建异种移植瘤或在体外培养，最终完成sgRNA测序。