Table 2 in Two new species of Ophiostomatales (Sordariomycetes) associated with the bark beetle Dryocoetes alni from Poland
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Table 2. Information on PCR primers used in this study. LocusPrimersFungiITS1-5.8SITS1-F (Gardes and Bruns 1993), ITS4 (White et al. 1990)Ceratocystiopsis, Leptographium28SLR0R, LR5 (Vilgalys and Hester 1990)CeratocystiopsisITS2-28SITS3 (White et al. 1990), LR3 (Vilgalys and Hester 1990)LeptographiumTUB2Bt2a, Bt2b (Glass and Donaldson 1995)CeratocystiopsisT10 (O’Donnell and Cigelnik 1997), Bt2b (Glass and Donaldson 1995)LeptographiumACTLepact-F, Lepact-R (Lim et al. 2004)LeptographiumCALCL3F, CL3R (De Beer et al. 2016)LeptographiumTEF1-αF-728F (Carbone and Kohn 1999), EF2 (O’Donnell et al. 1998)CeratocystiopsisEF1F, EF2R (Jacobs et al. 2004)Leptographium
表2 本研究中使用的聚合酶链式反应(Polymerase Chain Reaction,PCR)引物信息。 1. 基因座:真菌内转录间隔区1-5.8S核糖体RNA基因(ITS1-5.8S rRNA),引物:ITS1-F(Gardes与Bruns,1993)、ITS4(White等,1990) 2. 基因座:长喙壳孢属(Ceratocystiopsis)、细粘束孢属(Leptographium)28S核糖体RNA基因(28S rRNA),引物:LR0R、LR5(Vilgalys与Hester,1990) 3. 基因座:长喙壳孢属内转录间隔区2-28S核糖体RNA基因(ITS2-28S rRNA),引物:ITS3(White等,1990)、LR3(Vilgalys与Hester,1990) 4. 基因座:细粘束孢属β微管蛋白基因(TUB2),引物:Bt2a、Bt2b(Glass与Donaldson,1995) 5. 基因座:长喙壳孢属β微管蛋白基因(TUB2),引物:T10(O’Donnell与Cigelnik,1997)、Bt2b(Glass与Donaldson,1995) 6. 基因座:细粘束孢属肌动蛋白基因(ACT),引物:Lepact-F、Lepact-R(Lim等,2004) 7. 基因座:细粘束孢属钙调蛋白基因(CAL),引物:CL3F、CL3R(De Beer等,2016) 8. 基因座:细粘束孢属翻译延伸因子1-α基因(TEF1-α),引物:F-728F(Carbone与Kohn,1999)、EF2(O’Donnell等,1998) 9. 基因座:长喙壳孢属翻译延伸因子1-α基因(TEF1-α),引物:EF1F、EF2R(Jacobs等,2004)



