Comparative expression of whole duodenum from intestinal Arx deficient mice and control littermates

The enteroendocrine lineage secretes hormones which direct the digestion, absorption, and motility of the intestine. Arx is a transcription factor expressed specifically in the enteroendocrine cells. With intestinal-specific loss of Arx, hormonal expression is disrupted. We used microarrays to determine the differential expression of genes from the whole duodenum after removal of Arx, a gene specifically expressed in the enteroendocrine lineage, in the intestinal epithelium compared to littermate controls at postnatal day 5. Whole duodenum was dissected from postnatal day 5 mice. RNA extraction was performed with Trizol and then RNA was hybridized on Agilent 2-color microarray.肠内分泌细胞系（enteroendocrine lineage）可分泌激素，调控肠道的消化、吸收与蠕动功能。Arx是一种特异性表达于肠内分泌细胞的转录因子（transcription factor）。当肠道上皮特异性缺失Arx时，激素的表达会发生紊乱。本研究利用基因芯片（microarrays）技术，对比出生后第5天的肠道上皮特异性敲除Arx（Arx为特异性表达于肠内分泌细胞系的基因）的小鼠与同窝对照小鼠的完整十二指肠组织，以筛选差异表达基因。实验取材为出生后第5天小鼠的完整十二指肠组织，采用Trizol法提取总RNA，随后将RNA样本进行安捷伦（Agilent）双色基因芯片杂交。