Significance of topological congruence between <i>H. erato</i> and <i>H. melpomene</i> phylogenies.

Phylogenies were estimated separately for the clades of H. erato and H. melpomene and in a combined analysis of the Heliconius. The significance of the cophylogeny mapping analyses was estimated based on minimising total reconstruction costs with Jane 3, either by randomising the leaf associations (column 2) or by randomizing the H. melpomene phylogeny (column 3). The significance of the pairwise distance correlation was calculated, at the root of the H. melpomene phylogeny (column 4) and at the root of the H. erato phylogeny (column 5), using TreeMap 3. Each p-value was estimated with 1000 Monte-Carlo replicates, and the 95% confidence upper bound was calculated for each using Wilson's score interval for binomial proportions [68].我们分别针对红带袖蝶（H. erato）与诗神袖蝶（H. melpomene）的支系构建系统发育树，并对袖蝶属（Heliconius）开展联合系统发育分析。共系统发育映射（cophylogeny mapping）分析的显著性通过软件Jane 3以最小化总重建成本的方式进行评估，具体采用两种随机化策略：一是随机化终端分类单元关联（第2列），二是随机化诗神袖蝶的系统发育树（第3列）。借助TreeMap 3软件，我们分别在诗神袖蝶系统发育树的根节点（第4列）与红带袖蝶系统发育树的根节点（第5列）处，计算成对距离相关性的显著性。所有p值均通过1000次蒙特卡洛重复抽样（Monte-Carlo replicates）进行估算，并针对每个结果采用二项分布比例的威尔逊得分区间（Wilson's score interval）计算95%置信上限[68]。