Altered light-dark phase-dependent behavioral responses and suprachiasmatic nucleus pathology in an α-synuclein rat model of Parkinson's disease

Description This dataset accompanies the study investigating circadian rhythm dysfunction and suprachiasmatic nucleus (SCN) pathology in α-synuclein-overexpressing transgenic rats as a preclinical model of Parkinson’s disease (DOI: 10.21203/rs.3.rs-7816986/v1). The dataset contains behavioral, histological, and quantitative analysis data obtained from three-month-old α-synuclein transgenic rats and wild-type littermate controls. Experiments were designed to assess light-dark phase-dependent modulation of motor and non-motor behaviors and to evaluate structural and cellular alterations within the SCN. Behavioral assessments include measures of: exploratory activity, locomotor behavior, sucrose preference, and olfaction-guided feeding performance across different light-dark cycle phases. Histological and immunohistochemical analyses include: α-synuclein accumulation, overall SCN cell density, neuronal markers, orexinergic fiber markers, and microglial markers (including Iba1-positive cell quantification). The dataset further includes correlation analyses linking α-synuclein pathology with neuroinflammatory and orexinergic alterations. The data support the conclusion that α-synuclein-overexpressing rats exhibit disrupted phase-dependent behavioral modulation and early SCN pathology prior to pronounced motor impairment, thereby representing a translational model for studying non-motor symptoms and circadian dysfunction in Parkinson’s disease. Dataset Contents The repository may include: raw behavioral measurements, processed quantitative data, microscopy-derived quantifications, statistical analysis tables, and supplementary documentation required for data interpretation and reuse. Experimental Model Species: Rat (Rattus norvegicus) Model: α-synuclein-overexpressing transgenic rats (SPRD-Tg(RP11-115D19)2Uhg) Control group: Wild-type littermates Age at analysis: 3 months Additional Information For additional information regarding the dataset, experimental procedures, or data processing, readers may contact the corresponding author at any time.数据集说明 本数据集随附于一项以α-突触核蛋白（α-synuclein）过表达转基因大鼠作为帕金森病（Parkinson’s disease）临床前模型的研究，该研究探究了其昼夜节律紊乱与视交叉上核（suprachiasmatic nucleus, SCN）病理变化（DOI: 10.21203/rs.3.rs-7816986/v1）。 本数据集包含取自3月龄α-突触核蛋白转基因大鼠及其野生型同窝对照大鼠的行为学、组织学与定量分析数据。本实验旨在评估运动与非运动行为受明暗周期阶段的调控效应，并分析视交叉上核内的结构与细胞改变。 行为学评估涵盖不同明暗周期阶段下的如下检测指标： - 探索活动 - 运动行为 - 蔗糖偏好实验 - 嗅觉引导摄食能力 组织学与免疫组织化学分析涵盖以下内容： - α-突触核蛋白聚集情况 - 视交叉上核总细胞密度 - 神经元标志物 - 食欲素能纤维标志物 - 小胶质细胞标志物（含Iba1阳性细胞定量分析） 本数据集还包含关联分析数据，用于探究α-突触核蛋白病理与神经炎症及食欲素能系统改变之间的相关性。 本数据支持如下结论：α-突触核蛋白过表达大鼠在出现显著运动功能障碍前，即已出现阶段依赖性行为调控紊乱与早期视交叉上核病理变化，因此该模型可作为研究帕金森病非运动症状与昼夜节律功能障碍的转化研究模型。 数据集内容 本数据集仓库可包含： - 原始行为学测量数据 - 经处理的定量数据 - 显微镜成像定量结果 - 统计分析表格 - 用于数据解读与复用的补充文档 实验模型 - 物种：大鼠（Rattus norvegicus） - 模型：α-突触核蛋白过表达转基因大鼠（SPRD-Tg(RP11-115D19)2Uhg） - 对照组：野生型同窝大鼠 - 分析时年龄：3月龄 补充信息 如需了解本数据集、实验流程或数据处理的更多详情，读者可随时联系通讯作者。