Dnitrification-synC1(plastic biofilm)On March 28, 2025, to investigate the metabolic mechanisms of the synthetic microbial community under 7% salinity conditions, metatranscriptomic sequencing was performed when the NO3--N absorption had not yet reached its peak, with three biological replicates for each sample. The specific procedure was as follows: The synthetic microbial community was resuspended in 7% salinity low-carbon 2216E medium to an OD600 of approximately 0.8, followed by the addition of 0 and 20 mg/L NO3--N. The culture was incubated at 25°C for 1 hour in a biochemical incubator, followed by centrifugation at 4500 r/min for 10 minutes to collect the bacterial pellets. The centrifuge tubes were quickly frozen in liquid nitrogen. The samples were preserved with dry ice and shipped to Novogene Bioinformatics (Beijing, China). Metatranscriptomic sequencing was completed based on the Novaseq 6000 sequencing system (library insert size 350 bp, paired-end sequencing length 150 bp), generating 3 Gb of data for each sample.
Dnitrification-synT1(marine plastic biofilm)On March 28, 2025, to investigate the metabolic mechanisms of the synthetic microbial community under 7% salinity conditions, metatranscriptomic sequencing was performed when the NO3--N absorption had not yet reached its peak, with three biological replicates for each sample. The specific procedure was as follows: The synthetic microbial community was resuspended in 7% salinity low-carbon 2216E medium to an OD600 of approximately 0.8, followed by the addition of 0 and 20 mg/L NO3--N. The culture was incubated at 25°C for 1 hour in a biochemical incubator, followed by centrifugation at 4500 r/min for 10 minutes to collect the bacterial pellets. The centrifuge tubes were quickly frozen in liquid nitrogen. The samples were preserved with dry ice and shipped to Novogene Bioinformatics (Beijing, China). Metatranscriptomic sequencing was completed based on the Novaseq 6000 sequencing system (library insert size 350 bp, paired-end sequencing length 150 bp), generating 3 Gb of data for each sample.
Dnitrification-syn-48h-7%To evaluate the compositional stability of the synthetic bacterial community, cultures were incubated under salinities of 7%, with or without the addition of 20 mg/L NO₃⁻-N, and maintained at 25 °C in a static biochemical incubator for 48 h. After incubation, samples were centrifuged at 4500 rpm for 10 min to collect cell pellets, which were then rapidly frozen in liquid nitrogen. The frozen samples were kept on dry ice and sent to Novogene Bioinformatics Technology Co., Ltd. (Beijing, China) for 16S rRNA gene amplicon sequencing. Sequencing was performed on the Illumina NovaSeq 6000 platform using a paired-end 250 bp (PE250) strategy, generating approximately 8 Mb of data per sample.The quality of the sequencing data was assessed using the NGS QC Toolkit (version 2.3.3; Patel & Jain, 2012) with default parameters. High-quality reads were subsequently processed using Parallel-META (version 3.5.3; Jing et al., 2017) with the required parameters specified for taxonomic classification, community diversity estimation, and structural analysis based on 16S rRNA gene sequences. The resulting data were used to calculate the relative abundances of the three component strains within the synthetic community.
Dnitrification-syn-24h-7%To evaluate the compositional stability of the synthetic bacterial community, cultures were incubated under salinities of 7%, with or without the addition of 20 mg/L NO₃⁻-N, and maintained at 25 °C in a static biochemical incubator for 24 h and 48 h. After incubation, samples were centrifuged at 4500 rpm for 10 min to collect cell pellets, which were then rapidly frozen in liquid nitrogen. The frozen samples were kept on dry ice and sent to Novogene Bioinformatics Technology Co., Ltd. (Beijing, China) for 16S rRNA gene amplicon sequencing. Sequencing was performed on the Illumina NovaSeq 6000 platform using a paired-end 250 bp (PE250) strategy, generating approximately 8 Mb of data per sample.The quality of the sequencing data was assessed using the NGS QC Toolkit (version 2.3.3; Patel & Jain, 2012) with default parameters. High-quality reads were subsequently processed using Parallel-META (version 3.5.3; Jing et al., 2017) with the required parameters specified for taxonomic classification, community diversity estimation, and structural analysis based on 16S rRNA gene sequences. The resulting data were used to calculate the relative abundances of the three component strains within the synthetic community.
Dnitrification-synT2(marine plastic biofilm)On March 28, 2025, to investigate the metabolic mechanisms of the synthetic microbial community under 7% salinity conditions, metatranscriptomic sequencing was performed when the NO3--N absorption had not yet reached its peak, with three biological replicates for each sample. The specific procedure was as follows: The synthetic microbial community was resuspended in 7% salinity low-carbon 2216E medium to an OD600 of approximately 0.8, followed by the addition of 0 and 20 mg/L NO3--N. The culture was incubated at 25°C for 1 hour in a biochemical incubator, followed by centrifugation at 4500 r/min for 10 minutes to collect the bacterial pellets. The centrifuge tubes were quickly frozen in liquid nitrogen. The samples were preserved with dry ice and shipped to Novogene Bioinformatics (Beijing, China). Metatranscriptomic sequencing was completed based on the Novaseq 6000 sequencing system (library insert size 350 bp, paired-end sequencing length 150 bp), generating 3 Gb of data for each sample.
Dnitrification-synC3(marine plastic biofilm)On March 28, 2025, to investigate the metabolic mechanisms of the synthetic microbial community under 7% salinity conditions, metatranscriptomic sequencing was performed when the NO3--N absorption had not yet reached its peak, with three biological replicates for each sample. The specific procedure was as follows: The synthetic microbial community was resuspended in 7% salinity low-carbon 2216E medium to an OD600 of approximately 0.8, followed by the addition of 0 and 20 mg/L NO3--N. The culture was incubated at 25°C for 1 hour in a biochemical incubator, followed by centrifugation at 4500 r/min for 10 minutes to collect the bacterial pellets. The centrifuge tubes were quickly frozen in liquid nitrogen. The samples were preserved with dry ice and shipped to Novogene Bioinformatics (Beijing, China). Metatranscriptomic sequencing was completed based on the Novaseq 6000 sequencing system (library insert size 350 bp, paired-end sequencing length 150 bp), generating 3 Gb of data for each sample.
Dnitrification-syn-24h-3.5%To evaluate the compositional stability of the synthetic bacterial community, cultures were incubated under salinities of 3.5% with or without the addition of 20 mg/L NO₃⁻-N, and maintained at 25 °C in a static biochemical incubator for 24 h. After incubation, samples were centrifuged at 4500 rpm for 10 min to collect cell pellets, which were then rapidly frozen in liquid nitrogen. The frozen samples were kept on dry ice and sent to Novogene Bioinformatics Technology Co., Ltd. (Beijing, China) for 16S rRNA gene amplicon sequencing. Sequencing was performed on the Illumina NovaSeq 6000 platform using a paired-end 250 bp (PE250) strategy, generating approximately 8 Mb of data per sample.The quality of the sequencing data was assessed using the NGS QC Toolkit (version 2.3.3; Patel & Jain, 2012) with default parameters. High-quality reads were subsequently processed using Parallel-META (version 3.5.3; Jing et al., 2017) with the required parameters specified for taxonomic classification, community diversity estimation, and structural analysis based on 16S rRNA gene sequences. The resulting data were used to calculate the relative abundances of the three component strains within the synthetic community.
Dnitrification-syn-48h-3.5%To evaluate the compositional stability of the synthetic bacterial community, cultures were incubated under salinities of 3.5% with or without the addition of 20 mg/L NO₃⁻-N, and maintained at 25 °C in a static biochemical incubator for 48 h. After incubation, samples were centrifuged at 4500 rpm for 10 min to collect cell pellets, which were then rapidly frozen in liquid nitrogen. The frozen samples were kept on dry ice and sent to Novogene Bioinformatics Technology Co., Ltd. (Beijing, China) for 16S rRNA gene amplicon sequencing. Sequencing was performed on the Illumina NovaSeq 6000 platform using a paired-end 250 bp (PE250) strategy, generating approximately 8 Mb of data per sample.The quality of the sequencing data was assessed using the NGS QC Toolkit (version 2.3.3; Patel & Jain, 2012) with default parameters. High-quality reads were subsequently processed using Parallel-META (version 3.5.3; Jing et al., 2017) with the required parameters specified for taxonomic classification, community diversity estimation, and structural analysis based on 16S rRNA gene sequences. The resulting data were used to calculate the relative abundances of the three component strains within the synthetic community.
Dnitrification-synT3(marine plastic biofilm)On March 28, 2025, to investigate the metabolic mechanisms of the synthetic microbial community under 7% salinity conditions, metatranscriptomic sequencing was performed when the NO3--N absorption had not yet reached its peak, with three biological replicates for each sample. The specific procedure was as follows: The synthetic microbial community was resuspended in 7% salinity low-carbon 2216E medium to an OD600 of approximately 0.8, followed by the addition of 0 and 20 mg/L NO3--N. The culture was incubated at 25°C for 1 hour in a biochemical incubator, followed by centrifugation at 4500 r/min for 10 minutes to collect the bacterial pellets. The centrifuge tubes were quickly frozen in liquid nitrogen. The samples were preserved with dry ice and shipped to Novogene Bioinformatics (Beijing, China). Metatranscriptomic sequencing was completed based on the Novaseq 6000 sequencing system (library insert size 350 bp, paired-end sequencing length 150 bp), generating 3 Gb of data for each sample.
北极低分辨率融池覆盖率数据集(2007年)依托科技部重点研发计划项目提出了一种改进的基于中低分辨率可见光数据的融池覆盖率像元线性解混算法,利用2007年的MODIS L3数据-MOD09A1数据获得了2007年5-8月覆盖全北极的12.5km融池覆盖率数据集。