Video S1 - Evaluation of Injectable Constructs for Bone Repair with a Subperiosteal Cranial Model in the Rat

The video presents a new subperiosteal model in the cranial bone of the rat. First, the anesthetized animal is placed in a prone position. Further, anesthesia is maintained with 2% isoflurane in oxygen delivered via a facemask. The cranial surface is shaved and disinfected. A surgical blade is used to expose the periosteum of frontal bone by a midline skin incision of approximately 1 cm long. Next, a blunt needle (21G) attached to one 1 mL syringe is inserted under the bone periosteum at 45° to the frontal bone surface. The needle is shifted for 15 mm towards the face direction. The periosteum is carefully elevated by the tip in left and right directions. Then, a volume of 200 µL injectable construct is placed into the subperiosteal space. Finally, the wound is closed by suturing the skin (4-0 suture, Ethilon™, Ethicon). (ZIP)