Uncovering the mechanisms of RPPH1 polyadenylation and exploring how non-canonical polyadenylation influences the RNA landscape in breast cancer

Alternative polyadenylation is a widespread gene regulation mechanism often overlooked for non-protein coding genes. Here, investigations into alternative polyadenylation of the non-coding gene RPPH1 was performed in breast cancer models. Our findings reveal two different mechanisms are involved in generating the alternative forms. We propose dysregulation of these mechanisms can result in alternative gene expression that is worth further therapeutic consideration. We used transcriptomics to provide further evidence of dysregulated polyadenylation, especially in mitochondrial RNA and suggest a link to the Warburg effect.