Human Reference Protein Interactome Mapping Project

The Human Reference Protein Interactome Mapping Project (HuRI) is a repository, created by the Center for Cancer Systems Biology (CCSB) at Dana-Farber Cancer Institute, which stores the data from the ongoing work on the first complete reference map of the human protein-protein interactome network. All pairwise combinations of human protein-coding genes are systematically being interrogated to identify which are involved in binary protein-protein interactions. HuRI has grown in several distinct stages primarily defined by the number of human protein-coding genes amenable to screening for which at least one Gateway-cloned Open Reading Frame (ORF) was available at the time of the project. Three proteome-scale human PPI datasets are available via HuRI. PPI datasets generated in systematic screens on smaller sets of our human ORF collection have also been included. Also included are all PPIs identified in screens using different protein isoforms from the same gene as generated by alternative splicing.

人类参考蛋白质相互作用组图谱计划（HuRI）是由达纳-法伯癌症研究所（Dana-Farber Cancer Institute）下属的癌症系统生物学中心（Center for Cancer Systems Biology, CCSB）创建的数据库，存储了首个完整人类蛋白质-蛋白质相互作用组网络参考图谱的持续研究相关数据。研究人员正系统性地筛查所有人类蛋白编码基因的两两组合，以鉴定其中参与二元蛋白质-蛋白质相互作用的组合。HuRI的发展历经多个明确划分的阶段，其划分依据主要为当时可用于筛选的人类蛋白编码基因数量——这类基因在项目开展时需至少拥有一个经Gateway克隆的开放阅读框（Open Reading Frame, ORF）。HuRI可提供三份蛋白质组规模的人类蛋白质-蛋白质相互作用（Protein-Protein Interaction, PPI）数据集；此外，本数据库还收录了针对本项目人类ORF文库较小基因集开展系统性筛选所得到的PPI数据集；同时收录了通过使用同一基因经可变剪接产生的不同蛋白质异构体进行筛选所鉴定出的全部PPI。

搜集汇总

背景与挑战

背景概述

该数据集由Dana-Farber癌症研究所的癌症系统生物学中心创建，旨在构建首个完整的人类蛋白质-蛋白质相互作用网络参考图，通过系统筛选人类蛋白质编码基因对来识别二元相互作用。它包含三个蛋白质组规模的PPI数据集，以及基于较小ORF集合和不同蛋白质亚型的筛选数据。