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资源简介：
Liquid-based method for isolation of total DNA.
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创建时间：
2020-06-01
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Development and optimization of a silica column-based extraction protocol for ancient DNA
Rapid and cost-effective retrieval of endogenous DNA from ancient specimens remains a limiting factor in palaeogenomic research. Many methods have been developed to increase ancient DNA yield, but mod
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Improving the recovery for dissolved eDNA State: A comparative analysis of isopropanol precipitation, magnetic bead extraction, and centrifugal dialysis
# Improving the Recovery for Dissolved eDNA State: A Comparative Analysis of Isopropanol Precipitation, Magnetic Bead Extraction, and Centrifugal Dialysis [https://doi.org/10.5061/dryad.z08kprrnk](htt
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Supplementary material 8 from: Marquina D, Roslin T, Łukasik P, Ronquist F (2022) Evaluation of non-destructive DNA extraction protocols for insect metabarcoding: gentler and shorter is better. Metabarcoding and Metagenomics 6: e78871. https://doi.org/10.3897/mbmg.6.78871
Lysis buffers and purification protocols
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Primer sequences and cycling conditions used for amplification.
1Amplification was performed in a GeneAmp 9700 PCR System (Applied Biosystems) by a 10 min incubation at 95°C, followed by 45 cycles of 301 s. at 95°C, 45 s. at 60°C and 60 s. at 72°C. The program was
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Purity (A260/280 and A260/230) values of DNA extracted for each sample type using the Qiagen kits and a newly developed HV-CTAB-PCI method.
Purity (A260/280 and A260/230) values of DNA extracted for each sample type using the Qiagen kits and a newly developed HV-CTAB-PCI method.
Figshare2023-06-07 更新40
