File S1 - A Simple and Non-Invasive Method for Nuclear Transformation of Intact-walled Chlamydomonas reinhardtii

Supporting Information. Method S1, List of materials and equipments. Text S1, Description of reagent setup. Table S1, Summary of the optimal conditions established for nuclear transformation of C. reinhardtii. Table S2, Limitations and its solution of transformation by aminoclay. Figure S1, Schematic representation of spreading friction equipment. Figure S2, Digital camera images of resultant transformant colonies. Figure S3, Schematic representation of the approximate unit structure of aminoclay ([H2N(CH2)3]8Si8Mg6O12(OH)4)). Figure S4, TEM images and energy dispersive X-ray (EDX) analysis of aminoclay. Figure S5, Mechanical properties of plates with increasing concentration of agar (1.5%, 3.0%, and 4.0%). Figure S6, Schematic diagram of an expression construct and Southern blot analysis. (DOC)